Recombinant Chymotrypsin

Animal Protein Free, No Virus Contaminant

Catalog Number:RCT10

CAS: 9004-07-3
EC: 3.4.21.1

Source: human chymotrypsin, expressed in E. Coli.

DESCRIPTION

Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

ADVANTAGES

Animal origin free：Recombinant human chymotrypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived chymotrypsin.

High purity：Recombinant Chymotrypsin provides increased specificity and eliminates contaminated other proteases activities found in enzymes purified from pancreas.

Stable：A sterile recombinant human chymotrypsin lyophilized decreases the contamination risks and chance of activity loss during transport and storage.

APPLICATION

Hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. Suitable for peptide mapping, fingerprinting, and sequence analysis.

MAIN FEATURES

Source	E. Coli
M.W.	26, 950 Da
Purified by	HPLC
Format	White lyophilized r
Specific activity	≥1000 unit/mg pro
Purity	> 95% by HPLC

UNIT DEFINITION: One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

RECOMMEND USAGE

Prepare 1-10mg/ml with 1ml 50mM HAc, used within 2 days, or stored below -20℃ after repacked

STORAGE INSTRUCTIONS

Recombinant Chymotrypsin lyophilized should be stored under 2℃-8℃in sealed container.It is stable within 24 months. After dissolved, it should be stored under -20℃.