Recombinant Trypsin

Animal Origin Free, No Virus Contaminant

Catalog Number:RPT0201

CAS: 9002-07-7
EC: 3.4.21.4

Source: Porcine trypsin, expressed in E.coli.

DESCRIPTION

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7.0-9.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF , aprotinin, soybean

trypsin inhibitor and by metal chelating agents, e.g.EDTA.It is typically isolated from the pancreas of animals.Animal-derived materials

are now subject to more strigent regulations, as authorities strive to ensure safer biologics and pharmaceuticals, such as in insulin and

vaccine production.

Recombinant porcine trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. Recombinant trypsin is expressed as active protease , with equivalent properties compared to native trypsin .It is manufactured under NSF ISO 9001-2008 and in accordance to good manufacturing practice (GMP) guidelines.No animal derived products are used in the fermentation, purification, or final formulation.

ADVANTAGES

Animal origin free:No animal-derived material is used during production.

Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant porcine trypsin provides increased specific activity no less than 3800 USP units/mg pro.

2) Reduce production costs while improving manufacturing progresses.

MAIN FEATURES

Source	E. Coli
Purified by	HPLC
Format	White lyophilized powder
Specific activity	≥3800 USP units/mg pro
Purity（RP-HPLC)	NLT 70% β-trysin, NMT 20% α-trypsin

UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

RECOMMEND USAGE

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.

STORAGE INSTRUCTIONS

Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.