Recombinant Bovine Enterokinase High Specificity, High Purity

Catalog Number:REK08

CAS:9017-74-8
EC:3.4.21.9

Source: bovine enterokinase, expressed in E. Coli

DESCRIPTION

YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.

ADVANTAGES

1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK)

2) No any other contaminated proteases, no non-specific cutting sites.

Recommend Usage Condition:

Cutting condition: 25mM Tris-HCl 8.0

Fusion protein concentration: 0.1-1mg/ml (total protein content: 0.5-1.0mg)

EK content: 1-2U

Temperature: 25℃

Time: overnight or 12h-16h for digestion.

If digesttion under 2℃-8℃, prolong the digestion time two folds, or more enzyme added to short the digestion time.

Common components influence the action of enterokinase

＞200mM imidazole or ＞200mM NaCl or ＞5% glycerin, the reaction may be effected.The following suggestions are given:

1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

2) If the dialysis is inconvenient, please dilute the sample to ＜100mM imidazole, ＜50mMNaCl, ＜5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.

MAIN FEATURES

Source	E.Coli
M.W.	25, 850 Da
Specific Activity	One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein.
Storage Temperature	-20°C or below.
Stability	Keep cool with blue ice during shipping. Remained stable at 25°C for one week without activity lost. No activity lost after 5 cycles of frozen-thawing.

PRODUCT INFORMATION

Product	Activity	Packaging	Manufacturer
Recombinant Enterokinase	≥5 u/μl	100u;500u;bulk	YaxinBio