Recombinant Proteinase K

Catalog Number:RPK09

Cas:39450-01-6

EC:3.4.21.14

Alternate names:Protease K, Endopeptidase K, Tritirachium alkaline proteinas

DESCRIPTION

A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).

ADVANTAGES

Format	Lyophilized
M.W	29.8kD
Purity	99%
Activity	30 units/mg
DNAses and RNAses	not detected

UNIT DEFINITION

One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5(color by Folin-Ciocalteu reagent).

RECOMMEND USAGE

Prepare recombinant Proteinase K with 50 mM Tris-HCl, 2 mM Calcium acetate.The optimum pH is 8.0.

STORAGE INSTRUCTIONS

Store lyophilized at -20°C. Aliquot the product after reconstitution to avoid repeated freezing thawing cycles.

HANDLING

Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.