Recombinant Human Trypsin

Animal Origin Free, No Virus Contaminant

Catalog Number:RHT03

CAS: 9002-07-7
EC: 3.4.21.4

Source: human trypsin, expressed in E. Coli.

DESCRIPTION

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH is pH 7.0 - 8.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.

Recombinant human trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

ADVANTAGES

Animal origin free:

The use of recombinant human trypsin eliminates the risk of virus presence, and of any other potential adventitious agents found in animal pancreas-derived trypsin. No animal derived products are used in the fermentation, purification or final formulation.

Recombinant human trypsin:The amino acid sequence is the same as the human trypsin 2.

Stable:A sterile recombinant human trypsin lyophilized eliminates the contamination risks and decreases the chance of activity loss in the transportation and storage.

High purity:

1) Recombinant human trypsin provides increased specificity and eliminates contaminating activities found in lower purity enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

3) Purity is more than 95% by HPLC.

MAIN FEATURES

Source	E. Coli
Purified by	HPLC
Format	White or White-like lyophilized powder
Specific activity	≥ 2500 USP u/mg pro.
Purity	≥ 95% by HPLC

UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

STORAGE INSTRUCTIONS

Recombinant human trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.