V8 Protease

Catalog Number:V813

CAS:66676-43-5

EC:3.4.21.19

Full Name:Endoproteinase Glu-C, from Staphylococcus aureus strain V8, V8 Protease

DESCRIPTION

Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues.The optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors are Phosphoric acid diisopropyl ester fluoride (DFP) and alpha 2 - macroglobulin and Na-p-tosyl-L-lysine chloromethyl ketone (TLCK).

SPECIFIC ACTIVITY

Approx.20U/mg at 25℃ with Z-Phe-Leu-Glu-4-nitranilide as the substrate(approx.500U/mg at 37℃ with casein as the substrate).

RECOMMEND USAGE

Cutting condition:PH 8.0~8.5

PACKAGING

1U/vial，50µg/vial，2mg/vial，

STORAGE INSTRUCTIONS

V8 protease should be stored under -20℃ in sealed container.

APPLICATION

Protein structure and sequence analysis